선충 억제 활성 식물 추출물의 선발

초록

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activityagainst Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of 5,000 μg/mlwere mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examineddaily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influenceon the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibitednematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and withjuveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

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