ISOLATION AND EXPRESSION ANALYSIS OF A HOMOLOG OF THE 14-3-3 EPSILON GENE IN THE DIAMONDBACK MOTH, Plutella xylostella

초록

A full-length 14-3-3 gene homolog (also referred to as the Px14-3-3 epsilon "epsilon" or Px14-3-3 epsilon gene) was cloned from cDNA of the diamondback moth, Plutella xylostella. The Px14-3-3 transcript is 789 nucleotides in length, and the predicted polypeptide is 263 amino acids in length, with a calculated molecular mass of 29.6 kDa. The Px14-3-3 gene contains the typical and predicted 14-3-3 domains and motifs. The amino acid sequence of the diamondback moth 14-3-3 gene is very similar to that of other insect epsilons (e) but not to other insect zetas (zeta). In particular, the protein sequence of the Px14-3-3 gene shows high identity to the Bombyx mori epsilon (96.2%). Western blot analysis using an antibody against Px14-3-3 epsilon verified the expression of 14-3-3 epsilon in the larval, pupal, and adult stages. The Px14-3-3 epsilon expression patterns in all the different tissue types were examined in the fourth instar larvae. Px14-3-3 epsilon was detected in every tissue examined, including the body fat, hemocytes, brain, gut, and cuticle. (C) 2010 Wiley Periodicals, Inc.

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