Molecular identification of coagulase-negative staphylococci by rpoB sequence typing

초록

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negativestaphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNSidentification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used formolecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and comparedthe results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methodswere more reliable for species identification; moreover, RSTing provided more molecular epidemiological information thanthat from 16S rRNA gene sequencing.

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