PCL/gelatin nanofibers incorporated with starfish polydeoxyribonucleotides for potential wound healing applications

초록

Conventional wound dressings have several shortcomings because they are unspecific and partially effec-tive for wound healing. To enhance the therapeutic efficiency of wound dressings, we extracted poly-deoxyribonucleotides (PDRN) from Patiria pectinifera, and characterized using absorbance-based analysis, chemical composition analysis, and electrophoresis. We evaluated their cytotoxicity and wound healing activity on human dermal fibroblast (HDF) and human keratinocytes (HaCaT). Next, we used elec-trospinning to fabricate polycaprolactone (PCL)/gelatin (Gel)/PDRN nanofibers for wound dressing appli-cation. The average fiber diameters of PCL (P), PCL/Gel (PG), and PCL/Gel/PDRN (PGP) nanofibers were 582.88 +/- 202.65, 435.65 +/- 149.87, and 334.63 +/- 98.09 nm, respectively. The biocompatibility of the nano-fibers was assessed using MTT assay and FDA/PI staining on HDF and HaCaT, no cytotoxicity was observed. In vivo experiments with full-thickness skin defect mouse models confirmed that the PGP nanofiber accelerated the initial wound healing process, as shown by wound closure analysis and histo-logical analysis. Our results suggest that the PGP nanofiber has potential as a biomaterial for wound dressing applications and skin tissue engineering. (c) 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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