Purification and enzyme property of a cell-wall lytic enzyme produced by Bacillus sp. LM-8 against Lactobacillus plantarum

초록

Purification and characterization of enzyme property of a cell-wall lytic enzyme against Lactobacillus plantarum were carried out. Final specific activity of purified enzyme was 5.8 units/mg and purity of the enzyme was increased 8.3 fold compared with the enzyme activity in culture broth. The molecular weight of purified enzyme was estimated to be 60,000 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. Optimal pH and temperature for the activity of this enzyme were 3.0 and 40°C, respectively. The cell-wall lytic enzyme activity was maintained at 30°C when treating the enzyme for 30 mins, whereas the activity was decreased to 80% of the maximum level at 40°C The enzyme activity exhibited good stability at the range of pH 4∼7.

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