Analysis of Gene Expression in Cyclooxygenase-2-Overexpressed Human Osteosarcoma Cell Lines

초록

Osteosarcoma is the most common primary bone tumor, generally affecting young people. While the etiology ofosteosarcoma has been largely unknown, recent studies have suggested that cyclooxygenase-2 (COX-2) plays a critical role inthe proliferation, migration, and invasion of osteosarcoma cells. To understand the mechanism of action of COX-2 in thepathogenesis of osteosarcoma, we compared gene expression patterns between three stable COX-2-overexpressing cell linesand three control cell lines derived from U2OS human osteosarcoma cells. The data showed that 56 genes were upregulated,whereas 20 genes were downregulated, in COX-2-overexpressed cell lines, with an average fold-change ＞ 1.5. Among theupregulated genes, COL1A1, COL5A2, FBN1, HOXD10, RUNX2, and TRAPPC2 are involved in bone and skeletal systemdevelopment, while DDR2, RAC2, RUNX2, and TSPAN31 are involved in the positive regulation of cell proliferation. Amongthe downregulated genes, HIST1H1D, HIST1H2AI, HIST1H3H, and HIST1H4C are involved in nucleosome assembly and DNApackaging. These results may provide useful information to elucidate the molecular mechanism of the COX-2-mediatedmalignant phenotype in osteosarcoma.

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