Optimization of the extraction process for fermented Rhus verniciflua stokes using response surface methodology

초록

Extraction conditions for extraction yield (Y (1) ), total phenolic content (TPC, Y (2) ), and inhibition rate in human gastric carcinoma cells (Y (3) ) were optimized using response surface methodology for fermented Rhus verniciflua stokes (FRV). Optimal extraction conditions for effective use of FRV as a biofunctional material in food and pharmaceutical industries were investigated. Independent variables were extraction temperature (X (1) =25-105A degrees C), extraction time (X (2) =4-20 h), and the solvent to solid ratio (X (3) =10-30 mL/g). A second order polynomial model satisfactorily fitted experimental data with coefficient of determination (R (2) ) values of 0.9613, 0.9851, and 0.8038, respectively, for Y (1) , Y (2) , and Y (3) . Optimal conditions for the highest extraction yield of 2.37%, a total phenol content (TPC) value of 113.75 mg of tannic acid equivalents (TAE)/g, and an inhibition rate of 72.61% were derived at X (1) =89.95A degrees C, X (2) =9.25 h, and X (3) =25 mL/g.

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