Effect of Calcium Activated Factor on Properties of Actin and Myosin of Rabbit Skeletal Muscle

초록

The effect of calcium activated factor (CAF) on enzymatic properties of actin and myosin was investigated. SDS polyacrylamide gel electrophoresis revealed that CAF did not degrade actin, but a slight degradation was found in myosin during CAF digestion, which might have been due to contaminated protease (s) in CAF preparation. No influence was found in EDTA ATPase of myosin and polymerization of G-actin during CAF digestion. However, heavy meromyosin (HMM) ATPase activating ability of actin was slightly decreased during CAF digestion. Although CAF digestion slightly decreased the biological activity of myofibrillar proteins, a single sarcomere prepared by CAF digestion is a useful model for studying muscle contraction because of its almost intact contractility. © 1980, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.