SSRFING: A BASIC computer program for DNA fingerprinting analysis with microsatellite SSR information

초록

Since SSR or microsatellite DNA profiles are a powerful tool in fingerprinting analysis, numerous SSR loci have already been developed and genetic data have been collected in many plant species. To facilitate the data processing, a computer program for plant DNA fingerprinting analysis was developed, and applied to identify modern soybean varieties. The SSRFING program can identify varieties with the same or different DMA profiles using single locus or combined loci information, select the best primer sets, and estimate the parameters which are frequently used, such as effective allele number, differentiation ratio, etc. The program consisting of main menu and different files, can run on any IBM-compatible computer under the DOS operating system as well as be applied to other molecular markers besides SSR for the same purposes.

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