Characterization of Genes Related to Cirsimaritin Biosynthesis From Cirsium pendulum Fisch

초록

Cirsium setidens Nakai and C. pendulum Fisch are Korean endemic thistle plants. Cirsimaritin is a major biocompound present in the genus Cirsium. We quantified cirsimaritin from the leaves of C. setidens Nakai and the stems and flowers of C. pendulum Fisch. Genes encoding the biosynthesis of cirsimaritin were identified from the transcriptomes of C. setidens Nakai. Gene Ontology analysis of the transcriptomes revealed that 5,000 transcripts were classified in the molecular function categories of cyclic compound binding proteins, transferase activity proteins, and heterocyclic compound binding proteins. More than 10,000 transcripts were identified in the biological process categories of organic metabolic process, primary metabolic process, cellular metabolic process, and biosynthetic process. Because chalcone synthase (CHS) and chalcone isomerase (CHI) are key enzymes in flavonoid biosynthesis, detailed phylogenetic and sequence analyses were carried out for these two enzymes. The phylogenetic analysis revealed that the CHS and CHI proteins of C. setidens were closely related to the CHS and CHI proteins of the artichoke thistle, Cynara carducunlus.

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