Anti-inflammatory effects of Stephania brachyandra extract in LPS-triggered inflammatory signaling; Stephania brachyandra 추출물의 LPS에 의한 염증 반응 억제효과

초록

Inflammation is a protective mechanism against various stimuli, such as infection, tissue damage, and lipopolysaccharide (LPS). LPS is an endotoxin mainly found in the cell walls of Gramnegative bacteria. It is recognized by toll-like receptor 4 (TLR4) of macrophages and plays important roles in secreting proinflammatory cytokines to protect host cells. However, an excessive inflammation causes damage to cells or tissues, leading to inflammatory diseases. To discover novel inflammatory regulators derived from natural product, native plant extract libraries existing in Asia were screened, and found Stephania brachyandra (S. brachyandra) as a candidate. Although S. brachyandra has traditionally been known to have antiinflammatory and antioxidant effects in Asia, there is no scientific evidence. In this study, we confirmed the antiinflammatory effect and signaling mechanism of S. brachyandra extract in the inflammation induced by LPS. LPS-induced expression of pro-inflammatory mediators, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by S. brachyandra extract in the macrophage cell line RAW264.7. Furthermore, the inhibitory effect of S. brachyandra extract on pro-inflammatory cytokine expression was accompanied by inhibiting the activities of nuclear factor-kappa B (NF-κB) and nuclear factor of activated T-cell (NFAT) transcription factors. Therefore, we suggest that S. brachyandra has the possibility of future development as a natural product candidate for regulating inflammation © 2022, The Microbiological Society of Korea

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