Antifungal Chitinase against Human Pathogenic Yeasts from Coprinellus congregatus

초록

The inky cap, Coprinellus congregatus, produces mushrooms which become autolyzed rapidly to generate black liquid droplets, in which no cell wall is detected by microscopy. A chitinase (Chi2) which is synthesized during the autolytic phase of C. congregatus inhibits the growths of Candida albicans and Cryptococcus neoformans up to 10% at the concentration of 10 mu g/ml, about 50% at concentration of 20 mu g/ml, and up to 95% at the concentration of 70 mu g/ml. Upon treatment these yeast cells are observed to be severely deformed, with the formation of large holes in the cell wall. The two yeast species show no growth inhibition at the concentration of 5 mu g/ml, which means the minimum inhibitory concentrations for both yeast species are 10 mu g/ml under these experimental conditions.

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