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Cloning and Functional Verification of the Candida milleri HIS3 Gene Encoding Imidazoleglycerol Phosphate Dehydratase
- Park, Eun-Hee;
- Kwun, Se-Young;
- Han, Seung-Ah;
- Lee, Jong-Sub;
- Kim, Myoung-Dong
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2초록
The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.
키워드
Candida milleri; imidazoleglycerol phosphate dehydratase; HIS3; SACCHAROMYCES-CEREVISIAE; TRANSLATIONAL CONTROL; HIS3 GENE; YEAST; TRANSFORMATION; GCN4; BIOSYNTHESIS; SEQUENCES; HISTIDINE; ACTIVATOR
- 제목
- Cloning and Functional Verification of the Candida milleri HIS3 Gene Encoding Imidazoleglycerol Phosphate Dehydratase
- 저자
- Park, Eun-Hee; Kwun, Se-Young; Han, Seung-Ah; Lee, Jong-Sub; Kim, Myoung-Dong
- 발행일
- 2012-10
- 유형
- Article
- 권
- 22
- 호
- 10
- 페이지
- 1441 ~ 1445