Transcriptome dataset for comparative analysis of differentially expressed genes between wild type and transgenic potato plants overexpressing Nuclear Factor Y subunit A7

초록

Nuclear Factor Y (NF -Y) is divided into three different types of subunits, A, B, and C. NF -Ys play crucial roles in plants for controlling gene expression associated with various developmental processes and abiotic stresses, but it is mostly unknown the downstream genes regulated by NF -Ys in plant. One of the potato NF -Y genes, StNF-YA7 , increased potato's drought tolerance when overexpressed under the control of constitutive CaMV 35S promoter. Therefore, it was of interest what genes are regulated by the increased expression level of StNF-YA7 . To investigate the downstream genes of StNF-YA7 , the transcriptome sequencing was carried out for four potato lines, including Solanum tuberosum L 'Superior' as wild type (WT), empty vector control (VC), and two StNF-YA7 overexpressor lines (designated to StNF-YA7 #19 & #26). The RNA sequencing data was produced by the Illumina NovaSeq 60 0 0 sequencing system. The number of total raw reads obtained from the RNA sequencing was 36.7 million for WT, 36.2 for VC, 29.3 for StNF-YA7 #19, and 29.5 million for StNF-YA7 #26, respectively. The length of total raw reads for each sample was between 5.92 Gb ( StNF-YA7 #19) and 7.42 Gb (WT), and after filtering raw quality reads, the total length was between 5.81 Gb ( StNF-YA7 #19) and 7.29 Gb (WT). Each filtered clear read set of four transcriptomes was mapped on the potato reference genome, SolTub_3.0, and the percentage of mapped reads ranged from 89.8 % (VC) to 90.3 % (WT). GC contents range between 43.01 % ( StNF-YA7 #19) and 42.44 % ( StNF-YA7 #26). Q20 quality score ranges between 98.63 % ( StNF-YA7 #26) and 98.74 % (VC). (c) 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC license ( http://creativecommons.org/licenses/by-nc/4.0/ )

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