Antioxidative constituents from the seeds of Cuscuta chinensis

초록

MeOH extract of Cuscuta chinensis seeds was fractionated with n-hexane, EtOAc and BuOH successively, and antioxidant activities were tested for all fractions using DPPH free radical scavenging method. In the tested fractions, EtOAc fraction showed high antioxidant activity (EC₅₀, 50 μg) From the EtOAc fraction, five compounds have been isolated. On the basis of spectral data, these compounds were identified as β-sitosterol, methyl 4-hydroxy-3,5-dimethoxycinnamate, β-sitosterol-3-O-β-D-glucopyranoside, caffeic acid, quercetin, kaempferol and calycopteretin. Among these compounds, β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside showed no antioxidant activity. EC₅₀ values of methyl 4-hydroxy-3,5-dimethoxycinnamate, caffeic acid, quercetin, kaempferol and calycopteretin were 0.6, 8, 19, 17 and 12 μg, respectively.

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