다양한 배양조건에 따른 Lactobacillus plantarum BG0001 배양액의 항균활성

초록

Lactic acid bacteria (LAB) has been known to produce various antimicrobial agents such as bacteriocin which causes cell growth restriction or death. LAB having antimicrobial activity was isolated and identified as Lactobacillus plantarum BG0001. The culture broth of L. plantarum BG0001 exhibited antimicrobial activity against the both of gram positive (Bacillus cereus, Staphylococcus aureus) and negative (Escherichia coli, Vibrio parahaemolyticus, Salmonella typhimurium) strains by paper disc diffusion assay. Since the optimization of fermentation conditions could promote antimicrobial activity, we compared the antimicrobial activity of L. plantarum BG0001 culture broth depending on fermentation temperatures. When L. plantarum BG0001 was fermented at 30oC, the lowest minimum inhibited concentration (9.92 mg/mL) was shown as compared to those from 32, 35, 37oC. When 100 mL/L of molasses and 80 mL/L of corn steep liquor (CSL) were used instead of MRS medium, the minimum inhibited concentration (MIC) was 17.36 mg/L which suggesting molasses and CSL could be used for the fermentation of L. plantarum BG0001 as an alternative fermentation medium.

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