Radioprotective Effect of HME-Mulberry Leaves against Electromagnetic Radiation-Induced Damage in Rats

초록

In this study, a solid dispersion containing mulberry leaves and additives was manufactured by hot-melt extrusion (HME) to develop a radioprotective agent against electromagnetic radiation. The prepared formulations were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and electrophoretic light scattering (ELS). The formation of regular dispersions was confirmed through SEM and TEM analysis. To explore the radioprotective effect, animals were randomly classified into four groups: normal control (NC), irradiation (IR), irradiation after administration with mulberry leaves (MR), and irradiation after administration with HME-mulberry leaves (HR). Each sample was orally administered at 100 mg/kg/day, and 7 Gy of 6 MV electromagnetic radiation (X-ray) was used once for the whole body. Lymphocytes seemed to recover in the HR group, but none of the groups recovered at a normal range by the seventh day after irradiation. There was also no change in all groups of the red blood cells. The spleen index tended to recover in the HR group compared to the IR group, which was consistent with histological analysis. The spleen of the radiation-exposed groups (IR, MR, and HR) showed relatively atrophied white pulp and decreased lymphocytes. These symptoms further increased on the third day after irradiation and gradually alleviated on the seventh day. Compared to the IR group, the MR and HR groups had less loss of lymphocytes caused by damage to the white pulp. Specifically, the HR group demonstrated a higher recovery rate than the MR group, confirming the potential of HME-Mulberry as a radioprotective agent. The study's findings suggest further studies on health supplements and medicines to supplement the current research.

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