바이오매스 자원으로서의 Lemna Paucicostata의 인공배양조건과 이화학적 특성에 관한 연구

초록

Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor(total volume 2,630cm³, working volume 2,240cm³) was operated at conditions of 6,250 lux and 28℃. Water flow affected the growth of Lemna P.: growth rate was very high(more than 1.1 d－¹) at a condition of no-water movement, but it was very low (less than 0.15 d－¹) when water moved slowly. The growth of Lemna P. was higher in 16h d－¹ light cycle than in 8 and 24h d－¹, and it was also severely affected by the initial NH₄-N levels of wastewater. The growth rate of Lemna p. was high in lower NH₄-N level, indicating that the growth rate is in inverse proportion to NH₄-N concentration in wastewater. However, the contents of crude protein(CP) of Lemna P. were proportional to the initial NH₄-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 NH₄-N mg L－¹ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and 100 mg L－¹ had similar protein contents to linseed(CP 35%), cottonseed(CP 38%) and soybean(CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of 16h d－¹ light cycle and less than 2mg L－¹ of NH₄-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high(more than 1.1 d－¹) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:1n9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

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