Growth inhibition of plant pathogenic fungi by a chitinase of Coprinellus congregatus

초록

Experiments for growth inhibition of two different plant pathogenic fungal species, Alternaria alternata (KCTC26781) and Fusarium graminearum ZO3639 (type culture) and ASR1R1 (isolate from rice), by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus were carried out. In liquid media, Chi2 (50 μg/ml) inhibited more than 90°/o of germination of A alternata spore, and the growth of each fungal strain was totally inhibited by the addition of Chi2 at the concentrations of 70 μg/ml. When 6 pg of Chi2 was added twice a day at the hyphal tip zone, both strains of F. graminearum showed growth inhibition as well as decreased hyphal branching. © 2012, The Microbiological Society of Korea.

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