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초록

The multiple sclerosis caused by multiple inflammatorydisease or immune system disorder, is usually treatedby interferon β through adjusting the abnormal immune reactions. For high production of human interferon β using recombinantE. coli, codon optimized and wild type genes were synthesized. When pET-15b or pET-21a vector was used as anexpression vector with each gene, there was no target proteinexpression. When pQE30 vector was used as an expressionvector, human interferon β was expressed by recombinant E. coli XL1-blue and E. coli JM109. Using the codon optimizedgene, the expression of human interferon β was slightly increasedas compared to that from wild type gene. However, mostof expressed human interferon β was insoluble form.

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