Antioxidant Activity of Fractions from 70% Methanolic Extract of Sonchus oleraceus L.

초록

The potential antioxidant activities of different fractions from a 70% methanolic (MeOH) extract of Sonchus oleraceus were assayed in vitro. All of the fractions exception of n-hexane showed a strong antioxidant activity, especially the ethyl acetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50=19.25 mu g/mL). The results of hydroxyl radical scavenging activity and a reducing power assay showed concentration dependence, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in the Studies. Additionally, the fractions' total phenolic (TP) contents was measured, phenolic compounds Such as tannic acid, p-coumatric acid, quercetin, epicathchin, and kaempferol being detected by high performance liquid chromatography (HPLC). Meanwhile, a regression analysis revealed a moderate-to-high correlation coefficient between the antiradical activity and the TP Contents, Suggesting that fractions obtained from the 70% MeOH extract of S. oleraceus are of-potential use as Sources Of antioxidant material.

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