Generation of a cold-adapted PRRSV with a nucleotide substitution in the ORF5 and numerous mutations in the hypervariable region of NSP2

초록

A cold-adapted porcine reproductive and respiratory syndrome virus (CA-VR2332) was generated from the modified live virus strain VR2332. CA-VR2332 showed impaired growth when cultured at 37 degrees C with numerous mutations ((S)731(F), (E)819(D), (G)975(E), and (D)1014(N)) in the hypervariable region of the NSP2, in which the mutation (S)731(F) might play a vital role in viral replication at 30 degrees C. Conserved amino acid sequences of the GPS protein suggests that CAVR2332 is a promising candidate for producing an effective vaccine against PRRSV infection. Further studies on replication and immunogenicity in vivo are required to evaluate the properties of CA-VR2332.

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