Genetic variation and trait-marker associations in a core collection of Perilla frutescens L. revealed by phenotypic traits and SSR markers

초록

Perilla frutescens is generally classified into two major cultivated varieties: P. frutescens var. frutescens and var. crispa. Weedy forms of both varieties exhibit high levels of genetic diversity and are regarded as important genetic resources that are currently preserved in gene banks. To facilitate the effective utilization of Perilla germplasm, this study performed both phenotypic characterization and simple sequence repeat (SSR) marker analysis on a core collection comprised of 232 accessions representing the three types of Korean Perilla germplasm. The genotypic coefficient of variation for the 10 quantitative traits ranged from 6.0% to 52.5%, while the phenotypic coefficient of variation ranged from 6.2% to 55.5%. The SSR markers revealed high genetic diversity, with an average of 7.7 alleles per locus. The average gene diversity and polymorphism information content values were 0.638 and 0.588, respectively. The analysis of molecular variance indicated that genetic variation within the Perilla types (82%) was higher than that among the Perilla types (18%). Marker-trait association analysis identified eight SSR markers significantly associated with six quantitative traits. Among these, three SSR markers (KNUPF11, KNUPF26, KNUPF32) were associated with one or more traits. These results will contribute to future Perilla research and breeding by promoting the integration of phenotypic and genotypic information.

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