Analysis of Antioxidant Activity and the Marker Compound of Chamaecrista nomame (Siebold) H. Ohashi Extract; 차풀 추출물의 항산화 활성 및 지표성분 분석

초록

This study sought to investigate the antioxidant activity and the bioactive compound of Chamaecrista nomame (Siebold) H. Ohashi extract prepared using two extraction solvents (hot water and 40% ethanol). To establish the standardized extraction conditions (extraction solvent) for Chamaecrista nomame (Siebold) H. Ohashi extract, the total phenol content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability were evaluated. We also validated an analytical method for the determination of the marker compound, luteolin by evaluating the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of the developed high-performance liquid chromatography (HPLC) method. Our results showed that the TPC and DPPH radical scavenging ability were significantly higher in the 40% ethanol extract than in the hot water extract. The extraction yield of the standardized 40% ethanol extract was 16.56%. Luteolin in 40% ethanol extract was found to be a bioactive compound. However, p-coumaric acid was not detectable. The correlation coefficient of the calibration curve for the determination of luteolin in Chamaecrista nomame (Siebold) H. Ohashi extract was 1.0000. The LOD and LOQ were 0.12 μg/mL and 0.36 μg/mL, respectively. The inter-day and intra-day precision values were 0.09∼2.50% and 0.21∼1.44%, respectively. The inter-day and intra-day accuracies were 99.57∼98.52, and 97.12∼99.11% respectively. Thus, the analytical method for the determination of luteolin in Chamaecrista nomame (Siebold) H. Ohashi extract was validated. © 2025 The Korean Society of Food Science and Nutrition.

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