HPLC-DAD를 이용한 귀비탕 중 6종 생리활성 물질의 동시분석법 확립

초록

Guibi-tang, a traditional herbal medicine, is used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. To develop an analysis method of simultaneous determination of six compounds, swertisin, decursinol, glycyrrhizin,6-gingerol, costunolide and decursin in Guibi-tang, a high performance liquid chromatography was used with diode array detector. Six bioactive components were separated on a SHISEIDO C18 column (5 µm, 4.6 mm I.D.×250 mm) with column temperature 30oC. The gradient elution was composed of water with 0.1% trifluoroacetic acid (TFA) and acetonitrile. UV wavelength was set at 230 nm, 254 nm and 330 nm, respectively. Calibration curve showed good linear regression (R 2>0.9999). The limits of detection (LOD) and the limits of quantification (LOQ) ranged in 0.03 - 0.23 µg/ml and 0.08 - 0.70 µg/ml, respectively. The RSD values of intra- and inter-day test were in the range of 0.03 ~ 0.96% and 0.01 ~ 1.46%, respectively. The evaluated results of accuracy test were varied from 92.28% ~ 105.14% with RSD < 1.60%. In conclusion, this developed simultaneous determination method was accuracy and sensitive to the quality evaluation of Guibi-tang.

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