Effect of vertical flow exchange on microbial community distributions in hyporheic zones

초록

The effect of the vertical flow direction of hyporheic flux on the bacterial community is examined. Vertical velocity change of the hyporheic zone was examined by installing a piezometer on the site, and a total of 20,242 reads were analyzed using a pyrosequencing assay to investigate the diversity of bacterial communities. Proteobacteria (55.1%) were dominant in the hyporheic zone, and Bacteroidetes (16.5%), Actinobacteria (7.1%) and other bacteria phylum (Firmicutes, Cyanobacteria, Chloroflexi, Planctomycetesm and unclassified phylum OD1) were identified. Also, the hyporheic zone was divided into 3 points - down welling point, mixed welling point and up welling point - through vertical hydraulic gradient, and the bacterial communities were compared and analyzed. When the species diversity index was additionally analyzed based on the pyrosequencing data, richness and diversity of species were observed to be highest in the order of mixed welling point >down welling point >up welling point. Hence, quantitative changes and characteristics of the hyporheic zone were analyzed to have an effect on the resident bacterial communities.

키워드