활성화된 단핵구 및 대식세포의 항원제시기능에 대한 Kaempferitrin의 조절 효과

초록

Kaempferitrin, isolated from Kenaf (Hibiscus cannabinus), was examined to evaluate its modulatory effects onantigen-presenting cel functions of macrophages/monocytes such as phagocytosis of foreign materials, up-regulation of co-stimulatory molecules (CD40, CD80 and CD86), adhesion molecule activation, and antigen processing and presentation.Kaempferitrin strongly blocked up-regulation of CD40, CD80 and CD86, but not pattern recognition receptor (PRR) (e.g.,β1-integrins), as asessed by cell-cell adhesion assay, required forT cell-antigen-presenting cell (APC) interaction. Furthermore, this compound did not block a simple activation of CD29, asassessed by cel-fibronectin adhesion assay. However, the compound did not diminish phagocytic uptake, an initial step forantigen processing, and ROS generation in RAW264.7 cells. In particular, to understand molecular mechanism of kaempfer-itrin-mediated inhibition, the regulatory role of LPS-induced signaling events was examined using immunoblotting analysis.Interestingly, this compound dose dependently suppressed the phosphorylation of IκBα, Src, Akt and Syk, demonstratingmay be involved in regulating APC function-relevant immune responses of macrophages and monocytes by regulating intra-cellular signaling.Keywords . kaempferitrin, macrophages, monocytes, antigen-presenting cell function, co-stimulatory molecules, adhe-sion molecules

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