Polyquaternium enhances the colloidal stability of chitosan-capped platinum nanoparticles and their antibacterial activity

초록

Here, for the first time, we have developed a novel green synthesis method where chitosan acts as a reducing agent and as a colloidal stabilizer, together with polyquaternium for the synthesis of platinum nanoparticles (PtNPs). It was observed that only chitosan-stabilized PtNPs (Ch@PtNPs) were stable up to pH 5, with a diameter of around 89 nm. The diameter of the Ch@PtNPs increased with the increase in pH, indicating the instability of Ch@PtNPs at neutral and alkaline mediums. However, when polyquaternium (PQ) (a cationic polymer) was added as a stabilizer along with chitosan, the diameter of chitosan/polyquaternium stabilized PtNPs (Ch/PQ@PtNPs), i.e. 87 nm, remained almost constant up to pH 9. Similarly, the pH-dependent decrease in the surface charge of Ch@PtNPs was also attenuated with the addition of polyquaternium. This indicates high colloidal stability of Ch/PQ@PtNPs in acidic, neutral, as well as alkaline mediums. It was observed that Ch/PQ@PtNPs exhibited high antibacterial activity against Staphylococcus aureus, as compared to uncapped PtNPs and Ch@PtNPs. Thus, the addition of PQ increases the antibacterial properties of Ch/PQ@PtNPs against Staphylococcus aureus by enhancing the stability of PtNPs at neutral pH.

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