HPLC analysis and antioxidant activity of Ulmus davidiana and some flavonoids

초록

In this study, we investigated the antioxidative activities of (-)-catechin (1), (-)-catechin-7-O-beta-D-apiofuranoside (2) and (-)-catechin-7-O-beta-D-xylopyranoside (3) in terms of their abilities to promote metal chelation, prevent lipid peroxidation, and inhibit DNA cleavage. In addition, high-performance liquid chromatography (HPLC) was used to quantify compounds (1-3) in each of various solvent extracts from Ulmus davidiana. Regarding metal-chelating activity, (-)-catechin (1) exhibited moderate activity at a concentration of 180 mu g/ml, with 57.5 +/- 0.03% of metal ions chelated. However, (-)-catechin-7-O-beta-D-apiofuranoside (2) and (-)-catecliin-7-O-beta-D-xylopyranoside (3) exhibited weak chelating activity. The values obtained for compounds (2 and 3) were comparable to that of alpha-tocopherol at a concentration of 180 mu g/ml (9.11 +/- 0.01 %). In addition, (-)-catechin-7-O-beta-D-xylopyranoside (3) showed inhibitory activity similar to alpha-tocopherol (inhibition of lipid peroxidation). Moreover, compounds (1-3) exhibited moderate protective activity against DNA cleavage. These results are consistent with our results for antioxidative activity. (C) 2009 Elsevier Ltd. All rights reserved.

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