Effects of Genotypes on In Vitro Maturation and Fertilization of Frozen-Thawed Porcine Oocytes

초록

In the present study, we carried out to investigate the effects of gene on in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes. The porcine cumulus-oocyte complexes (COCs) were divided into four groups according to whether they were: (1) in vitro matured; (2) cryopreserved, and then matured in vitro; (3) in vitro fertilized and (4) cryopreserved, and then in vitro fertilized. Maturation of porcine COCs was accomplished by incubation in NCSU23 medium. The cryopreservation method of immature oocytes was Open Pulled Straws (OPS) developed by Vajta et al. (1998). Oocytes stained by Acetic-Orcein method were observed the status under the microscope. DNA extracting from the ovaries was analyzed by RAPD (Random amplified polymorphic DNA) and SSCP (single strand conformational polymorphism) methods. The rates of oocytes maturation and fertilization were significantly high for AA genotype. The results indicated that in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes may be affected by gene in pigs.

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