발효된 도라지 추출물의 지표성분(Platycoside E 및 Platycodin D) 분석법 검증

초록

The aim of this study was to validate an analytical method for the determination of platycoside E and platycodin D in Platycodon grandiflorum root extract. Specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of platycoside E and platycodin D were measured by high performance liquid chromatography. Correlation coefficients of the calibration curve for platycoside E and platycodin D were 0.9999 and 0.9997, respectively. The inter-day and intra-day precision values of platycoside E and platycodin D ranged from 0.58 to 3.59% and from 0.4 to 4.77%, respectively. The inter-day nd intra-day accuracies were 94.02 to 115.73% and 94.11 to 122.82%, respectively. The LOD for platycoside E and platycodin D was 0.22 μg/mL and 0.31 μg/mL, respectively. The results validated the analytical determination of platycoside E and platycodin D in Platycodon grandiflorum root extract.

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