The Importance of FACS Analysis in the Development of Aptamers Specific to Pathogens

초록

Purpose: This review aims to introduce aptamers and the methods of its development to improve the sensitivity andselectivity to target bacteria. In this review, we have highlighted current developments and directions in the pathogendetection based on aptamers. Background: Aptamers, the specific nucleic acid sequences, can bind to targets with highaffinity and specificity. Some of researches on the use of aptamers for the detection of pathogen have been reported inrecent years. Aptamers have more applicability than antibodies for the development of pathogen detection using biosensor;such as easy to synthesis and labeling, lack of immunogenicity, and a low cost of production. However, only few reports onthe development and use of aptamers for the detection of pathogen have been published. Review: Aptamers specific topathogen are obtained by whole-cell systematic evolution of ligands by exponential enrichment (SELEX) process. SELEXprocess is composed of screening random oligonucleotide bound with target cells, multiple separation and amplification ofnucleic acids, final identification of the best sequences. For improving those affinity and selectivity to target bacteria,optimization of multiple separating process to remove unbounded oligonucleotides from aptamer candidates and sortingprocess by flow cytometry are required.

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