Cell-type specificity of interleukins lot and 1α and 1β prostaglandin and plasminogen activator production in bovine endometrial cells

초록

Interleukin (IL)-1 alpha is a potent stimulator of prostaglandin production in bovine endometrium, and IL-1 affects plasminogen activator (PA) activity in several types of cells. In this study, we determined the effects of IL-1 alpha and IL-1 beta on production of the prostaglandins PGF(2 alpha) and PGE(2) and on PA activity in cultured bovine endometrial epithelial and stromal cells. We also determined the effects of PGE(2) and PGF(2 alpha) on PA activity in these cells. Finally, we used RT-PCR to examine the expression of IL-1 alpha, IL-1 beta, and IL-1 receptor type 1 (IL-1R) mRNA in cultured bovine endometrial cells. This analysis revealed that IL-1 alpha mRNA was present only in the stromal cells, whereas IL-1 beta and IL-1R mRNAs were present in both cell types. When cultured cells were exposed to IL-1 alpha and IL-1 beta at concentrations ranging from 0.006 to 3 nM for 24 h, IL-1 alpha and IL-1 beta were found to dose-dependently stimulate PGE(2) and PGF(2 alpha) production in stromal cells (P < 0.05) but not in epithelial cells. On the other hand, exposure to IL-1 alpha and IL-1 beta dose-dependently increased PA activity in the epithelial cells, whereas neither stimulated PA production in the stromal cells. When cells were exposed to IL-1 alpha and IL-1 beta at concentrations ranging from 0.06 to 3 nM for 24 h, the two IL-1s differed in their effects on both PGE(2) and PGF(2 alpha) production in stromal cells and had significantly differed in their effects on PA activity in epithelial cells. Exposure to PGE(2) and PGF(2 alpha) did not affect PA activity in either stromal or epithelial cells (P > 0.05). Taken together, these results suggest the possibility that both IL-1 alpha and IL-1 beta are produced by the stromal cells, that IL-1 beta is produced by the epithelial cells, and that IL-1 alpha is a far more potent stimulator than IL-1 beta of prostaglandin and PA production in cultured bovine endometrial epithelial and stromal cells. (c) 2008 Elsevier B.V. All rights reserved.

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