Assessment of β-Lactamase Inhibitor Potential of Medicinal Plant Extracts against Antibiotic-resistant Staphylococcus aureus

초록

This study was designed to assess the possibility of using medicinal plant extracts as β-lactamase inhibitors tocontrol antibiotic-resistant Staphylococcus aureus. The susceptibilities of S. aureus ATCC 15564 (SAWT), ciprofloxacininduced S. aureus ATCC 15564 (SACIP), oxacillin-induced S. aureus ATCC 15564 (SAOXA), and clinically-isolated S. aureus CCARM 3008 (SACLI) to ampicillin were determined in the absence and presence of medicinal plant extracts,including Cleyera japonica (CJ), Carpinus laxiflora (CL), Euphorbia helioscopia (EH), Euscaphis japonica (EJ),Oenothera erythrosepala (OE), and Rosa multiflora (RM). The phenotypic change in the clear inhibition zones aroundampicillin disc was observed for SAWT, SACIP, and SAOXA, indicating the production of ampicillinase. Compared to thecontrols, the MICs of ampicillin against SAWT, SACIP, and SAOXA were decreased from 4 to 0.5 ㎍/mL in the presence of CL,16 to 4 ㎍/mL in the presence of RM, and 32 to 2 ㎍/mL in the presence of CL, EH, and RM, respectively. The medicinalplant extracts, OE, EJ, and CL, effectively inhibited the β-lactamase activities of SAWT (78%), SACIP (57%), and SAOXA(76%) when compared to the control. This results suggest that the medicinal plant extracts can be used as BLIs to control theantibiotic-resistant S. aureus.

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