대기부유분진추출물로 야기된 DNA 손상에 대한 Quercetin, Vitamin C 및 Trolox의 보호효과

초록

In order to evaluate the genotoxicity of airborne particulate matter extracted with dichloromethane (APE), therat microsome mediated (S-9) or DNA repair enzyme treated Comet assays were performed using the single cell gel elec-trophoresis in A549 human lung carcinoma cells. It was found that the cells interacting with APE showed more DNA single-strand breaks relative to untreated cells. The genotoxicity of APE was increased with the treatment of S-9 mixture.Microsome mediated DNA damage was inhibited by CYP1A1 inhibitor, quercetin. The APE also showed oxidative DNAmin C and Trolox. We also found that the vegetables or fruits extract may reduce APE-induced genotoxicity by their anti-oxidant activity and CYP1A1 inhibition.Keywords . airborne particulate matter, Comet asay, DNA damage, CYP1A1 inhibitor, antioxidant, vegetable, fruit

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