Screening of biologically active essential oils from Ligusticum tenuissimum

초록

The biological activities of the crude essential oils from Ligusticum tenuissimum and the control (phthalic anhydride) were compared. About 60% of the growth of MCF7, A549, and Hep3B cells were inhibited by adding 1.0 mg/ml of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell (IMR90) was scored as 34.4% for the crude oil and 26.4% for control, respectively. It was found that the crude essential oils were more effective than the control in anti mutagenicity tested by both Rec-assay and CHO V79 cells. The growth of human T-cell (Jurkat) was enhanced up to 1.21 times by adding the crude essential oil compared with the control. 50% of α-glucosidase activity was inhibited by both the crude essential oil and the control. ACE activities were inhibited 80.1% and 65.3% by adding 1.0 mg/ml of the crude oil and the control, respectively. The higher enhancement of glutathione-S-transferase activity was observed in the crude oil than those in the control: 301% v.s 234% at 1.0 mg/ml of the treatment. Thrombolytic activity was measured as 42.9% and 28.6% for the crude oil and the standard, respectively. The effect of the oil on the nerve cells PC12, was observed as follows: the neurite of PC12 cells was lengthened up to 255 μm longer than 205 μm of control. The number of neurite-bearing cells were about two times higher than control. The survival ratio of the crude essential oil was also increased up to 56.4% which was about two fold higher than in control.

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