염분과 저온에 대한 내성증진을 위한 GST 유전자의 과발현

초록

Cotton Glutathione S-Transferase (GST: EC 2.5.1.18) was cloned and overexpressed in tobacco (Nicotiana tabacum) plants. Northern blot analysis confirmed the successful transformation of cotton gst gene in tobacco plant. Type I and Type II transcript patterns were identified in transgenic tobacco plants and only Type I transcripts were discussed in this paper. The activity of GST in the type II transgenic plants was about 1.5-fold higher than those of the wild type and non-expresser by using 1-chloro-2,4-dinitrobenzene (CDNB) and reduced glutathione as the substrate. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type II transgenic plants produced functional GST in the cells. The effects of cotton GST in the seedlings was evaluated by growing the control and transgenic seedlings at 15°C in the growth chamber in the light. Overexpressors were grown well compared to the control plants (non-expressors). To test for tolerance to salinity, seeds of Gh-5 overexperessors and the wild type Xanthi seedlings were grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings on 50 and 100 mM NaCL solution. There was no difference in growth raet at 150 and 200 mM NaCl concentration.

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