Effects of mixed extract from Lycium Chinense, Cordyceps militaris, and Acanthopanax senticosus on glucose-regulating enzymes of HepG2 in hyperglycemic conditions

초록

This study investigated the anti-diabetic effects of the mixed water extract (JDG 100) composed of Lycii Cortex, Acanthopanax senticosus and Cordyceps militaris on glucose-regulating key enzymes such as glucokinase (GK), acetyl-CoA carboxylase (ACC). In the current study, HepG2 cells were exposed to pathological condition such as hyperglycemic condition (4.5 g glucose/L) with JDG 100 and then experiments such as RT-PCR and Western blotting were carried out. JDG 100 treated cells increased to 168±0.04% and 182.4±0.03% in GK mRNA and protein expressions, respectively, compared to control. Treatment of the JDG 100 up-regulated ACC mRNA (127.3±0.02%) and protein (126.7±0.24%) of HepG2 cells in the high glucose media. These observations suggest that JDG 100 mixed water extract may have a potential as an anti-diabetic agent in type 2 diabetes mellitus.

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