Regulation of Thioltransferase Activity from Schizosaccharomyces pombe

초록

Thioltransferase (TTase), also known as glutaredoxin (Grx), is an enzyme catalyzing the reduction of a variety of disulfide compounds and acting as a cofactor for various enzymes such as ribonucleotide reductase. The Schizosaccharomyces pombe cells, exponentially grown in rich medium at 30°C, were shifted to 20°C and 35°C. The yeast cells, shifted to 35°C, showed higher TTase activity than the cells continuously grown at 30°C, whereas the yeast cells, shifted to 20°C, gave lower TTase activity. The S. pombe cells, exponentially grown in minimal medium and shifted from 30°C to 35°C and 40°C, produced higher TTase activity. When the S. pombe cells were initially incubated in rich and minimal media at three different temperatures (25°C, 30°C and 35°C), they showed higher TTase activity at higher temperature. These results suggest that the TTase activity of S. pombe is regulated by temperature.

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