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초록
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons. (C) 2009 Elsevier Masson SAS. All rights reserved.
키워드
Dammarenediol synthase; Centella asiatica; Oxidosqualene cyclase; BETA-AMYRIN SYNTHASE; OXIDOSQUALENE CYCLASE; CYCLOARTENOL SYNTHASE; LUPEOL SYNTHASE; TRITERPENE; BIOSYNTHESIS; CLONING; EXPRESSION; ENZYME; GENE
- 제목
- Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban
- 저자
- Kim, Ok Tae; Lee, Jei Wan; Bang, Kyong Hwan; Kim, Young Chang; Hyun, Dong Yun; Cha, Seon Woo; Choi, Yong Eui; Jin, Mei Lan; Hwang, Baik
- 발행일
- 2009-11
- 유형
- Article
- 권
- 47
- 호
- 11-12
- 페이지
- 998 ~ 1002