Inhibitory effect of ultrasonicated sesame meal on fat accumulation of 3T3-L1 adipocytes and oleic acid induced HepG2 hepatocytes

초록

Antioxidant activities of an aqueous extract of sesame meal (SM -HW) and an ultrasonicated sesame meal (SSMHW) were evaluated and anti-obesity activity of SSM-HW was determined using in vitro assays. In addition, metabolite analyses were conducted using GC/MS to determine major non-volatile compounds in SM -HW and SSM-HW. Antioxidant activities of SSM-HW were significantly higher than those of SM -HW. SSM-HW had higher concentrations of some organic acids and amino acids than SM -HW. Differentiation of 3T3 -L1 adipocytes was decreased by 20 % after treatment with 10 mu g/mL SSM-HW compared to control. Expression levels of several adipocyte marker genes and proteins were significantly decreased compared to control ( p < 0.05) when 3T3 -L1 adipocytes were treated with SSM-HW. In addition, SSM-HW decreased fat accumulation in HepG2 hepatocytes and expression of adipocyte marker genes and proteins. Overall, SSM-HW had improved antioxidant power and effectively inhibited fat accumulation in 3T3 -L1 adipocytes and oleic acid-induced HepG2 hepatocytes.

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