국내 토끼풀에서 분리한 Cucumber mosaic virus의 특성

초록

A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showingmosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified throughconfirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysisof coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco speciesincluding Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. InChenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMVTr1compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5%homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP geneindicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is thefirst report of CMV in T. repens in Korea.

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