Application of NHS-dextran-based protein arrays for analysis of blood proteins with a spectral SPR biosensor

초록

In general, antibody arrays were favored to analyze proteins in blood samples or cell extracts because the arrays may provide selective immobilization of target proteins. However, in this study, we proposed protein arrays based on amide-linked (AL) NHS-dextran as an alternative approach to analyze C-reactive protein with a spectral SPR biosensor. The surface modification with AL NHS-dextran was based on amide linkage between amine-modified gold arrays and NHS-modified CM-dextran. The AL NHS-dextran arrays were characterized with FT-IR spectroscopy and atomic force microscopy (AFM). Moreover, AL NHS-dextran arrays were compared with the previously used epoxide-linked carboxymethyl dextran. In order to analyze quantitatively C-reactive protein in human sera on protein arrays, we have investigated the effect of buffer pH on the binding affinity of proteins to the arrays. Then, we have successfully analyzed C-reactive protein in human sera on the dextran arrays. Thus, protein arrays based on the AL NHS-dextran surface can be used as alternative method for rapid analysis of blood proteins.

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