산겨릅나무 줄기추출물의 생리활성 및 유효성분 분리

초록

Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity (RC50 = 3.15㎍/㎖) among the fractions. The isolated compounds were analyzed by 1H and 13C NMR, IR, UV/VIS, MS spectrum data and identified as catechin, ρ-Hydroxyphenethyl alcohol 1-O-ß-D-(6"-O-galloyl)-glucopyranoside. The compounds have shown strong antioxidant activity, with similar activity to BHA (RC50 = 2㎍/㎖). Especially, ρ-Hydroxyphenethyl alcohol 1-O-ß-D-(6"-O-galloyl)- glucopyranoside was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in 125㎍/㎖. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting 63㎍/㎖ of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.

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