Impact of an anti-Salmonella. TyphimuriumBacteriophage on intestinal microbiota and immunity status of laying hens

초록

Typhoid is a distinct gastrointestinal disease that largely affects the public by consumption of inadequately or partially cooked eggs from contaminated laying hen farms. This has led the research on laying hens to focus on controlling the contamination by an effective anti-Salmonellaspp. agent in the intestine. The treatments included, control, without challenge; PC,Salmonella typhimuriumchallenged (STC); BP5, 5 ppm bacteriophage/kg + STC; BP10, 10 ppm bacteriophage/kg + STC, onSalmonellashedding, body organs inflammatory reactions, and expression of toll-like receptor (TLR), pro-inflammatory cytokines, and heat shock protein (HSP) in the jejunum, liver,and thigh muscle in the STC laying hens. The RT-PCR method was used to enumerate the number ofSalmonella typhimuriumin the organs. The birds in the STC groups exhibited the increased population ofSalmonellaspp. in the excreta (p < .01). In the STC groups, the BP5 and BP10 laying hens exhibited a lower (p < .01) population ofSalmonellaspp. in the excreta at d 7 after STC. Supplementation of bacteriophage significantly decreased (p < .01) the colonization ofS. Typhimuriumin the spleen, oviduct, caecum and excreta. Among the STC treatments, the BP10 laying hens showed lower (p < .01) mRNA expression of interferon-gamma (IFN gamma) and TLR-4 in the jejunum compared with the PC treatment. After the STC, dietary supplementation with BP5 or BP10 decreased (p < .01) the mRNA expressions of IFN gamma, HSP-27 and tumour necrosis factor-alpha in the liver compared with the PC treatment. These results suggest that bacteriophage can be used as an effective agent to decreaseS. Typhimuriumcontamination in laying hens and possibly lowerS. Typhimuriumtransfer to foods.

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