Genetic diversity and population structure of Canna edulis accessions in Vietnam revealed by ISSR markers

초록

Discovery of the genetic diversity of Canna edulis accessions is valuable for germplasm conservation, improvement, and utilization of this crop as an ornamental garden plant or a food source in the future. This study employed 14 ISSR markers and morphological characters to assess genetic relationships among 52 accessions of Canna edulis (denoted as C1 to C52) collected in 9 provinces of three agroecological regions of Vietnam. A total of 75 loci with an average of 5.2 loci per marker and sizes ranging from 300 to 1900 bp were obtained. Most of the loci were polymorphic with an average polymorphism of 81.88% and a PIC value of 0.735. The Jaccard’s similarity coefficients were in the range of 0.42 and 0.98. The UPGMA analysis grouped the 52 accessions into two groups at the cut-off value of 0.54 with 13 and 39 accessions respectively. Population structure analysis also indicated a similar pattern of grouping for two sub-populations. Genetic variation occured within the population (90%). Additionally, the leaf width and the plant height were the two key morphological traits explaining the differences between the two sub-populations, suggesting for use of these traits in the classification and taxonomy of Canna edulis in Vietnam. © The Author(s) under exclusive licence to Society for Plant Research 2023.

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