Expression Pattern of Two Dehydroascorbate Reductase Genes from Tomato (Solanum lycopersicum L.) in Response to Stress

초록

Dehydroascorbate reductase (DHAR) plays an important role in the ascorbate-glutathione cycle in plants by controlling the homeostasis of reactive oxygen species. Expression profiles of two DHAR genes (SIDHAR1 and SIDHAR2) in tomato leaves were analyzed in response to abiotic and oxidative stresses as well as their expression in various organs were investigated under normal growth conditions. Multiple copies of SIDHAR homologues exist in the tomato genome. SIDHAR1 expression was dominant in stems and flowers but moderate in leaves; however, SIDHAR2 induction was strong in stems, leaves, and flowers, and young leaves showed the highest expression of SIDHAR2. Both genes were transcribed after treatment with salt, dehydration, and methyl viologen. Prolonged stress reduced SIDHAR genes expression. The level of hydrogen peroxide increased, and DHAR activity was altered by stress treatment. Results indicate that SIDHAR genes could play a role in multiple stress responses.

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