Proteomic and Genetic Diversity of Mycoplasma hyopneumoniae Isolates from South Korea

초록

The purpose of the present study was to evaluate the diversity of Mycoplasma hyopneumoniae (Mhp) isolates from South Korea. Overall, 42 field isolates recovered from 36 herds were analyzed using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and random amplification of polymorphic DNA (RAPD). Fifteen highly virulent strains were identified based on the presence of a 181 kDa band, a protein band associated with high virulence. A variability of 25.3% and 35.5% was noticed between field strains and J strain at protein and genomic level, respectively. Among the field strains, a variability of 22.2% and 31% was observed at protein and genomic level, respectively. Furthermore, isolates from the same herd demonstrated differences in protein and RAPD patterns. These results reveal high proteomic and genetic diversity among the field isolates of South Korea.

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