Cloning of the LEU2 gene from the amylolytic yeast Saccharomycopsis fibuligera

초록

The SfLEU2 structural gene of LEU2 encoding beta-isopropylmalate dehydrogenase was cloned from the amylolytic yeast Saccharomycopsis fibuligera isolated from Nuruk, a Korean traditional starter for rice wine production. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 1,107 bp, including a stop codon, encoding a 368 amino acid residue. The deduced amino acid sequence of Leu2 in S. fibuligera was 76.4% homologous with Candida boidinii and Kluyveromyces marxianus sequences. The SfLEU2 gene complemented leu2 Delta mutation in Saccharomyces cerevisiae, suggesting that the gene encodes a functional LEU2 in S. fibuligera. The GenBank Accession No. for SfLEU2 is KR820563.

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