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초록

Neurofilament light chain (NFL), one of the subunits of neurofilaments present inside neurons, is known as a biomarker of nerve axonal damage in early stage of Alzheimer's disease. Measuring the level of NFL is being actively studied worldwide because it can determine the extent of damage to nerve axons, especially in the early stages of Alzheimer's disease. However, ELISA based NFL detection techniques have several limitations because of expensive equipment and high cost. Herein, we perform the application of antibody conjugated Gold nanoparticles (ACNP) to detect NFL conveniently and efficiently. First, to conjugate Gold nanoparticles and antibody, carboxyl groups are modified on the particle surface to react the amine group of the antibody using EDC/ NHS. Then, the prepared ACNP react with the NFL protein to detect NFL. ACNP were characterized by DLS, ELS, Microplate Reader and TEM. Detected NFL by ACNP was confirmed by Western blotting, and the detection efficiency and limit of detection were confirmed through the absorbance change. This platform for detecting NFL using ACNP, which provides a cheap, selective, and convenient response, is a promising tool for diagnosis of neuronal damage through NFL detection in the physiological-pathological range.

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